| Final | time | L | seed | ______ | | 1. South Africa | 3:53.93 | 4 | 3:48.27 | | Karin Prinsloo, Natasha de Vos, Roxanne Tammadge, Suzaan Van Biljon | | 2. Zimbabwe | 3:57.81 | 7 | | | Kirsty Coventry, Samatha Welsh, Kirsten Lapharm, Nicole Horn | | 3. Algeria | 4:02.84 | 5 | 3:54.00 | | Fella Benaceur, Melia Bekhouche, Sarah Abdelrahmane, Amel Melih | | 4. Mozambique | 4:10.10 | 3 | 4:11.40 | | Jessica Vieira, Mariam Corsini, Faina Salate, Gessica Stagno | | 5. Nigeria | 4:18.42 | 6 | 4:20.15 | | Doutimi Gagbe, Faith Edorodion, Racheal Tonjor, Ifiezibe Gagbe | | 6. Kenya | 4:20.39 | 2 | 4:40.98 | | Sylvia Brunlehner, Talisa Lanoe, Sonia Cege, Soraya Oruya |
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| Record - 2003 | | South Africa 3:52.74 |
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