| Final 17:00 | time | | L | React. | 50m | birth | | 1. Farida Osman |  EGY | 55.41s | AGR,NR | 5 | 0.74 | 26.73s 1. | | | 2. Karin Prinsloo |  RSA | 55.69s | | 4 | 0.81 | 27.19s 2. | 1989 | | 3. Rwan Elbadry | EGY | 57.03s | | 3 | 0.75 | 27.72s 3. | | | 4. Majda Chebaraka |  ALG | 57.94s | | 2 | 0.76 | 28.35s 6. | 2000 | | 5. Marlies Ross | RSA | 57.94s | | 6 | 0.73 | 27.95s 4. | 1997 | | 6. Farah Ben Khelil |  TUN | 59.51s | | 7 | 0.84 | 28.01s 5. | 1998 | | 7. Sylvia Brunlehner |  KEN | 59.72s | | 1 | 0.74 | 28.53s 7. | 1994 | | 8. Tarryn Rennie |  ZIM | 1:00.81 | | 8 | 0.69 | 28.74s 8 | 1995 |
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| | | Tarryn Rennie (ZIM) replaced Naomi Ruele (BOT). |
| Ranking | time | | 1. Karin Prinsloo | RSA | 55.86s | 1. H2 | | 2. Farida Osman | EGY | 56.91s | 1. H1 | | 3. Rwan Elbadry | EGY | 57.11s | 2. H2 | | 4. Marlies Ross | RSA | 57.66s | 3. H2 | | 5. Chebaraka Majda | ALG | 58.59s | 4. H2 | | 6. Naomi Ruele |  BOT | 59.12s | 5. H2 | | 7. Farah Ben Khelil | TUN | 59.75s | 2. H1 | | 8. Sylvia Brunlehner | KEN | 59.84s | 3. H1 | | eliminated | | 9. Heather Arseth |  MRI | 1:00.13 | 4. H1 | | 10.Tarryn Rennie | ZIM | 1:00.49 | 6. H2 | | 11.Emily Muteti | KEN | 1:00.58 | 5. H1 | | 12.Ifiezibe Gagbe |  NGR | 1:01.90 | 7. H2 | | 13.Ana Nobrega |  ANG | 1:02.17 | 6. H1 | | 14.Christa Kaimansa Sowah |  GHA | 1:07.97 | 7. H1 | | 15.Stefan Bellore Sangala |  CGO | 1:19.10 | 8. H2 |
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