| Final 18:00 | Team | time | | L | React. | Bib | | 1. Nery Brenes Cardenas | CRC | 44.65s | PB | 1 | 0.194 | 519 | | 2. Luguelin Santos Aquino | DOM | 44.71s | PB | 6 | 0.263 | 564 | | 3. Ramon Samuel Miller | BAH | 45.01s | SB | 5 | 0.209 | 427 | | 4. Williams Collazo | CUB | 45.33s | | 4 | 0.161 | 529 | | 5. Noel Ruiz | CUB | 45.69s | | 7 | 0.363 | 549 | | 6. Arismendy Peguero | DOM | 45.72s | | 2 | 0.216 | 561 | | 7. Christopher Devon Brown | BAH | 45.89s | | 3 | 0.162 | 423 | | 8. Anderson Henriques | BRA | 45.92s | | 8 | 0.190 | 458 |
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| World Record: | | Michael Johnson (USA) 43.18s | | Seville (ESP) 26.08.1999 | | Panamerican Record: | | Ronald Ray (USA) 44.45s | | México City (MEX) 18.10.1975 |
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| Heats - Tuesday 25.10 - First 2 and Best progress. |
| Heat 1 16:45 | Team | time | | L | React. | Bib | | 1. Williams Collazo | CUB | 45.70s | | 7 | 0.187 | 529 | | 2. Anderson Henriques | BRA | 45.71s | | 8 | 0.171 | 458 | | 3. Arismendy Peguero | DOM | 45.94s | | 2 | 0.210 | 561 | | 4. Erison Hurtault | DMA | 46.24s | | 5 | 0.214 | 556 | | 5. Alberto Aguilar | VEN | 46.39s | | 1 | 0.257 | 769 | | 6. Orlando Garcia | MEX | 47.45s | | 3 | 0.170 | 652 | | 7. Richard Richardson | ANT | 48.49s | | 6 | 0.270 | 402 | | 8. Keron Toussaint | GRN | 1:37.36 | | 4 | 0.184 | 584 |
| Heat 2 16:52 | Team | time | | L | React. | Bib | | 1. Ramon Samuel Miller | BAH | 45.40s | | 4 | 0.203 | 427 | | 2. Luguelin Santos Aquino | DOM | 45.41s | PB | 6 | 0.235 | 564 | | 3. Nery Brenes Cardenas | CRC | 45.42s | | 7 | 0.199 | 519 | | 4. Winston George | GUY | 46.93s | | 5 | 0.225 | 596 | | 5. Geiner Alonso Mosquera | COL | 46.97s | SB | 2 | 0.296 | 513 | | 6. Annert Whyte | JAM | 47.57s | | 3 | 0.205 | 638 | | 7. Tremaine Ryan Alexander Harris | CAN | 48.21s | | 8 | 0.177 | 477 | | 8. Jayson Jones | BIZ | 51.60s | | 1 | 0.168 | 439 |
| Heat 3 16:59 | Team | time | | L | React. | Bib | | 1. Christopher Devon Brown | BAH | 45.92s | | 7 | 0.199 | 423 | | 2. Noel Ruiz | CUB | 45.98s | | 2 | 0.296 | 549 | | 3. Michael Mason | JAM | 46.09s | | 8 | 0.177 | 627 | | 4. Joshua Scott | USA | 46.09s | | 6 | 0.235 | 757 | | 5. Omar Longart | VEN | 46.50s | | 3 | 0.205 | 776 | | 6. Takeshi Salvador Fujiwara | ESA | 46.92s | | 5 | 0.225 | 579 | | 7. Augusto Jose Stanley | PAR | 47.52s | | 4 | 0.203 | 676 |
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