| Final A | Team | time | -time | L | React. | 50m | | 1. Rachel Bootsma | USA | 1:00.37 | RP | 5 | 0.60 | 1. | 29.56 | | 2. Elizabeth Anne Pelton | USA | 1:01.12 | 0.75 | 4 | 0.66 | 2. | 29.87 | | 3. Fernanda Gonzalez | MEX | 1:02.00 | 1.63 | 3 | 0.63 | 4. | 30.04 | | 4. Fabiola Molina | BRA | 1:02.04 | 1.67 | 6 | 0.71 | 3. | 30.03 | | 5. Carolina Colorado | COL | 1:02.83 | 2.46 | 1 | 0.63 | 7. | 31.01 | | 6. Gabrielle Soucisse | CAN | 1:02.88 | 2.51 | 7 | 0.74 | 5. | 30.25 | | 7. Gisela Maria Morales | GUA | 1:03.37 | 3.00 | 2 | 0.65 | 6. | 30.36 | | 8. Isabella Arcila | COL | 1:03.80 | 3.43 | 8 | 0.64 | 8. | 31.08 |
| Final B | Team | time | -time | L | React. | 50m | | 9. Cecilia Bertoncello | ARG | 1:03.26 | | 4 | 0.54s | 2. | 30.54s | | 10.Samantha Nicole Corea | CAN | 1:04.03 | 0.77s | 6 | 0.57s | 4. | 31.12s | | 11.Florencia Perotti | ARG | 1:04.78 | 1.52s | 3 | 0.68s | 7. | 31.48s | | 12.Alana Dillette | BAH | 1:04.85 | 1.59s | 7 | 0.58s | 5. | 31.17s | | 13.Etiene Medeiros | BRA | 1:04.85 | 1.59s | 5 | 0.63s | 1. | 30.27s | | 14.Estela Davis | MEX | 1:05.28 | 2.02s | 1 | 0.59s | 6. | 31.34s | | 15.Jeserik Pinto | VEN | 1:05.38 | 2.12s | 2 | 0.66s | 3. | 30.88s | | 16.Kiera Leigh Aitken | BER | 1:06.12 | 2.86s | 8 | 0.62s | 8. | 32.41s |
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| World record: | | Gemma Spofforth (GBR) 58.12s | | Roma (ITA) 28.07.2009 | | Pan American Games record: | | Barbara Bedford (USA) 1:01.71 | | Mar del Plata (ARG) 14.03.1995 |
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| Qualification: | | Standard A: 1:05.3 | | Standard B: 1:07.3 |
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| Heats | Team | time | -time | L | React. | 50m | Heat | | 1. Elizabeth Anne Pelton | USA | 1:01.57 | RP | 4 | 0.76s | 1. | 29.94s | 2. | | 2. Rachel Bootsma | USA | 1:01.75 | 0.18s | 4 | 0.59s | 1. | 29.95s | 3. | | 3. Fernanda Gonzalez | MEX | 1:01.84 | 0.27s | 5 | 0.60s | 2. | 30.13s | 3. | | 4. Fabiola Molina | BRA | 1:02.81 | 1.24s | 4 | 0.68s | 1. | 30.31s | 1. | | 5. Gisela Maria Morales | GUA | 1:02.95 | 1.38s | 3 | 0.66s | 3. | 30.21s | 3. | | 6. Gabrielle Soucisse | CAN | 1:03.04 | 1.47s | 5 | 0.73s | 2. | 30.34s | 2. | | 7. Carolina Colorado | COL | 1:03.22 | 1.65s | 6 | 0.63s | 4. | 30.63s | 3. | | 8. Isabella Arcila | COL | 1:04.30 | 2.73s | 6 | 0.75s | 3. | 30.88s | 2. | | 9. Cecilia Bertoncello | ARG | 1:04.53 | 2.96s | 6 | 0.57s | 2. | 31.03s | 1. | | 10.Etiene Medeiros | BRA | 1:04.59 | 3.02s | 5 | 0.61s | 3. | 31.09s | 1. | | 11.Florencia Perotti | ARG | 1:04.94 | 3.37s | 2 | 0.67s | 6. | 31.56s | 3. | | 12.Samantha Nicole Corea | CAN | 1:05.01 | 3.44s | 7 | 0.59s | 5. | 31.49s | 3. | | 13.Jeserik Pinto | VEN | 1:05.27 | 3.70s | 2 | 0.65s | 4. | 31.35s | 1. | | 14.Alana Dillette | BAH | 1:05.34 | 3.77s | 3 | 0.74s | 4. | 31.58s | 2. | | 15.Estela Davis | MEX | 1:05.50 | 3.93s | 7 | 0.66s | 5. | 31.70s | 2. | | 16.Kiera Leigh Aitken | BER | 1:05.63 | 4.06s | 3 | 0.61s | 5. | 31.74s | 1. | | 17.Alana Berrocal | PUR | 1:06.09 | 4.52s | 2 | 0.73s | 6. | 31.76s | 2. | | 18.Karen Vilorio | HON | 1:07.06 | 5.49s | 1 | 0.75s | 7. | 32.56s | 2. | | 19.Kendese Nangle | JAM | 1:07.47 | 5.90s | 7 | 0.59s | 6. | 32.36s | 1. | | 20.Ines Remersaro | URU | 1:07.61 | 6.04s | 1 | 0.72s | 7. | 32.31s | 3. | | 21.Laura Rodriguez | DOM | 1:09.51 | 7.94s | 1 | 0.60s | 7. | 32.95s | 1. |
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