| Final A | Team | time | -)time | L | React. | 50m | 100m | 150m | | 1. Julia Elizabeth Smit | --- | 2:13.73 | | 4 | 0.73s | 4. | 29.83s | 2. | 1:02.62 | 1. | 1:42.13 | | 2. Alia Atkinson | --- | 2:14.75 | 1.02 | 5 | 0.73s | 2. | 28.89s | 4. | 1:05.08 | 2. | 1:42.57 | | 3. Joanna Maranhao | --- | 2:15.08 | 1.35 | 3 | | 1. | 28.61s | 3. | 1:03.59 | 4. | 1:44.23 | | 4. Whitney Barrett Myers | --- | 2:15.23 | 1.50 | 7 | 0.68s | 3. | 29.17s | 1. | 1:02.36 | 3. | 1:43.69 | | 5. Hanna Pierse | --- | 2:19.12 | 5.39 | 6 | 0.78s | 6. | 30.55s | 5. | 1:05.50 | 5. | 1:45.63 | | 6. Arantxa Medina | --- | 2:21.74 | 8.01 | 8 | | 5. | 30.40s | 6. | 1:07.88 | 6. | 1:48.58 | | 7. Georgina Bardach | --- | 2:22.65 | 8.92 | 2 | | 7. | 30.68s | 7. | 1:07.93 | 7. | 1:48.98 | | 8. Daniela Victoria | --- | 2:22.67 | 8.94 | 1 | | 8. | 30.80s | 8. | 1:09.33 | 8. | 1:49.71 |
| Final B | Team | time | -)time | L | React. | 50m | 100m | 150m | | 9. Mckayla Lightbourn | --- | 2:20.62 | | 2 | | 1. | 30.05 | 2. | 1:06.95 | 1. | 1:47.80 | -. | | 10.Paige Schultz | --- | 2:21.38 | 0.76 | 4 | | 1. | 30.05 | 1. | 1:06.27 | 2. | 1:49.30 | -. | | 11.Julia Arino | --- | 2:24.01 | 3.39 | 5 | 0.54 | 4. | 30.82 | 3. | 1:07.03 | 3. | 1:50.87 | -. | | 12.Eliana Barrios | --- | 2:25.01 | 4.39 | 6 | | 5. | 31.07 | 5. | 1:08.46 | 5. | 1:52.49 | -. | | 13.Larissa Cieslak | --- | 2:26.23 | 5.61 | 3 | | 6. | 31.26 | 4. | 1:07.82 | 4. | 1:52.04 | -. | | 14.Zara Bailey | --- | 2:26.84 | 6.22 | 8 | 0.80 | 8. | 31.85 | 7. | 1:10.37 | 8. | 1:53.55 | -. | | 15,Ana Castellanos | --- | 2:27.31 | 6.69 | 7 | 0.71 | 7. | 31.36 | 8. | 1:10.75 | 6. | 1:53.04 | -. | | 16.Barbara Caraballo | --- | 2:27.57 | 6.95 | 1 | 0.71 | 3. | 30.53 | 6. | 1:08.54 | 7. | 1:53.12 | -. |
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| World record: | | Pan American Games record: |
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| Qualification: | | Standard A: 2:25.7 | | Standard B: 2:30.1 |
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| Heats | Team | time | -)time | L | React. | 50m | 100m | 150m | Heat | | 1. Julia Elizabeth Smit | --- | 2:15.52 | | 4 | 0.77s | 2. | 30.51s | 1. | 1:03.63 | 1. | 1:42.90 | Heat 3. | | 2. Alia Atkinson | --- | 2:18.04 | 2.52s | 5 | 0.74s | 1. | 29.17s | 2. | 1:06.82 | 2. | 1:44.83 | Heat 3. | | 3. Joanna Maranhao | --- | 2:19.71 | 4.19s | 5 | | 2. | 30.20s | 2. | 1:05.48 | 1. | 1:46.69 | Heat 2. | | 4. Hanna Pierse | --- | 2:19.86 | 4.34s | 5 | | 3. | 31.45s | 1. | 1:06.64 | 1. | 1:45.25 | Heat 1. | | 5. Georgina Bardach | --- | 2:20.57 | 5.05s | 3 | | 3. | 30.57s | 4. | 1:07.02 | 3. | 1:46.45 | Heat 3. | | 6. Whitney Barrett Myers | --- | 2:20.62 | 5.10s | 4 | 0.64s | 1. | 30.12s | 1. | 1:03.89 | 2. | 1:46.99 | Heat 2. | | 7. Daniela Victoria | --- | 2:22.04 | 6.52s | 3 | | 2. | 31.25s | 4. | 1:09.32 | 2. | 1:49.96 | Heat 1. | | 8. Arantxa Medina | --- | 2:23.08 | 7.56s | 6 | 0.79s | 5. | 31.09s | 5. | 1:08.21 | 4. | 1:48.87 | Heat 3. | | 9. Paige Schultz | --- | 2:23.31 | 7.79s | 4 | | 1. | 31.13s | 2. | 1:07.43 | 3. | 1:51.09 | Heat 1. | | 10.Julia Arino | --- | 2:23.74 | 8.22s | 2 | | 6. | 31.38s | 3. | 1:06.95 | 5. | 1:50.51 | Heat 3. | | 11.Larissa Cieslak | --- | 2:25.57 | 10.05s | 6 | 0.77s | 4. | 31.75s | 3. | 1:08.33 | 4. | 1:52.18 | Heat 1. | | 12.Eliana Barrios | --- | 2:25.97 | 10.45s | 7 | | 4. | 30.89s | 6. | 1:09.45 | 6. | 1:53.00 | Heat 3. | | 13.Mckayla Lightbourn | --- | 2:26.66 | 11.14s | 3 | | 3. | 30.30s | 3. | 1:07.43 | 3. | 1:51.65 | Heat 2. | | 14.Ana Castellanos | --- | 2:28.03 | 12.51s | 7 | 0.74s | 7. | 32.46s | 6. | 1:12.17 | 5. | 1:53.47 | Heat 1. | | 15.Barbara Caraballo | --- | 2:28.36 | 12.84s | 6 | 0.75s | 4. | 30.75s | 4. | 1:08.87 | 4. | 1:53.84 | Heat 2. | | 16.Zara Bailey | --- | 2:28.38 | 12.86s | 2 | 0.78s | 5. | 32.16s | 5. | 1:11.23 | 6. | 1:54.35 | Heat 1. | | 17.Maria Alejandra Torres | --- | 2:30.53 | 15.01s | 1 | 0.76s | 7. | 32.33s | 7. | 1:10.08 | 7. | 1:57.00 | Heat 3. | | 18.Lisa Blackburn | --- | 2:31.87 | 16.35s | 7 | 0.80s | 6. | 32.28s | 6. | 1:13.55 | 5. | 1:56.02 | Heat 2. | | 19.Daniella Van Den Berg | --- | 2:32.43 | 16.91s | 8 | | 8. | 33.87s | 8. | 1:13.68 | 8. | 1:59.60 | Heat 3. | | 20.Daniela Reyes | --- | 2:33.37 | 17.85s | 1 | | 6. | 32.43s | 7. | 1:13.34 | 7. | 1:58.26 | Heat 1. | | 21.Lara Maria Butler | --- | 2:34.86 | 19.34s | 1 | 0.74s | 5. | 32.07s | 5. | 1:11.31 | 6. | 1:57.76 | Heat 2. | | -. Alana Dillette | --- | DNS | | 2 | | | | | | | | Heat 2. | | -. Laura Rodriguez | --- | DNS | | 8 | | | | | | | | Heat 2. |
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