| Final 12.08 10:31 | Team | time | L | Rk. | | 1. Aaron Peirsol | USA | 52.54s | 2 | 3. 5. | | 2. Matt Grevers | USA | 53.11s | 5 | 1. 2. | | 3. Arkady Vyatchanin | RUS | 53.18s | 3 | 2. 3. | | 3. Hayden Stoeckel | AUS | 53.18s | 4 | 7. 1. | | 5. Ashley Delaney | AUS | 53.31s | 8 | 9. 8. | | 6. Liam Tancock | GBR | 53.39s | 7 | 6. 6. | | 7. Aschwin Wildeboer | ESP | 53.51s | 6 | 4. 4. | | 8. Junichi Miyashita | JPN | 53.99s | 1 | 10.7. |
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| Semi 1 11.08 10:57 | Team | time | L | Rk. | | 1. Arkady Vyatchanin | RUS | 53.06s | 4 | 3. | | 2. Aschwin Wildeboer | ESP | 53.51s | 5 | 4. | | 3. Liam Tancock | GBR | 53.61s | 3 | 6. | | 4. Junichi Miyashita | JPN | 53.69s | 2 | 7. | | 5. Tomomi Morita | JPN | 53.95s | 7 | 10. | | 6. Gregor Tait | GBR | 54.37s | 8 | 12. | | 7. Lubos Krizko | SVK | 54.38s | 6 | 13. | | 8. Mirco di Tora | ITA | 54.92s | 1 | 15. |
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| Semi 2 11.08 11:01 | Team | time | L | Rk. | | 1. Hayden Stoeckel | AUS | 52.97s | 6 | 1. | | 2. Matt Grevers | USA | 52.99s | 4 | 2. | | 3. Aaron Peirsol | USA | 53.56s | 5 | 5. | | 4. Ashley Delaney | AUS | 53.76s | 2 | 8. | | 5. Markus Rogan | AUT | 53.80s | 1 | 9. | | 6. Gerhard Zandberg | RSA | 53.98s | 3 | 11. | | 7. Stanislav Donets | RUS | 54.57s | 7 | 14. | | 8. Guy Barnea | ISR | 54.93s | 8 | 16. |
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