| Final 19:38 | time | +time | L | | | 1. Michael Phelps |  USA | 51.21 | | 04 | Stats | | 2. Chad le Clos |  RSA | 51.44 | +0.23s | 05 | Stats | | 2. Evgeny Korotyshkin |  RUS | 51.44 | +0.23s | 08 | Stats | | 4. Milorad Cavic |  SRB | 51.81 | +0.60s | 06 | Stats | | 4. Steffen Deibler |  GER | 51.81 | +0.60s | 07 | Stats | | 6. Joeri Verlinden |  NED | 51.82 | +0.61s | 02 | Stats | | 7. Tyler McGill | USA | 51.88 | +0.67s | 03 | Stats | | 8. Konrad Czerniak |  POL | 52.05 | +0.84s | 01 | Stats |
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 | | Michael Phelps |
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| Semi 1 | time | +time | L | Rk. | | | 1. Michael Phelps | USA | 50.86s | | 04 | 1. | Stats | | 2. Steffen Deibler | GER | 51.76s | +0.90s | 03 | 6. | Stats | | 3. Konrad Czerniak | POL | 51.78s | +0.92s | 05 | 7. | Stats | | 4. Chris Wright |  AUS | 52.11s | +1.25s | 02 | 11. | Stats | | 5. Jiawei Zhou |  CHN | 52.30s | +1.44s | 06 | 13. | Stats | | 6. Bence Pulai |  HUN | 52.40s | +1.54s | 07 | 14. | Stats | | 6. Benjamin Starke | GER | 52.40s | +1.54s | 08 | 14. | Stats | | 8. Francois Heersbrandt |  BEL | 52.71s | +1.85s | 01 | 16. | Stats |
| Semi 2 | time | +time | L | Rk. | | | 1. Chad le Clos | RSA | 51.42s | | 04 | 2. | Stats | | 2. Tyler McGill | USA | 51.61s | +0.19s | 06 | 3. | Stats | | 3. Milorad Cavic | SRB | 51.66s | +0.24s | 03 | 4. | Stats | | 4. Joeri Verlinden | NED | 51.75s | +0.33s | 02 | 5. | Stats | | 5. Evgeny Korotyshkin | RUS | 51.85s | +0.43s | 05 | 8. | Stats | | 6. Dinko Jukic |  AUT | 51.99s | +0.57s | 01 | 9. | Stats | | 7. Nikolay Skvortsov | RUS | 52.03s | +0.61s | 07 | 10. | Stats | | 8. Jason Dunford |  KEN | 52.16s | +0.74s | 08 | 12. | Stats |
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| | S | F | | Semi 1 | | 3 | | Semi 2 | | 5 | | Heat 6 | 6 | 3 | | Heat 5 | 4 | 3 | | Heat 4 | 4 | 2 | | Heat 3 | 2 | | Heat 2 | | Heat 1 |
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| Heat 6 | time | +time | L | Rk. | | | 1. Michael Phelps | USA | 51.72s | | 04 | 2. | Stats | | 2. Milorad Cavic | SRB | 51.90s | +0.18s | 05 | 5. | Stats | | 3. Joeri Verlinden | NED | 52.07s | +0.35s | 06 | 9. | Stats | | 4. Chris Wright | AUS | 52.11s | +0.39s | 03 | 10. | Stats | | 5. Nikolay Skvortsov | RUS | 52.12s | +0.40s | 02 | 11. | Stats | | 6. Francois Heersbrandt | BEL | 52.22s | +0.50s | 01 | 13. | Stats | | 7. Takeshi Matsuda |  JPN | 52.36s | +0.64s | 08 | 16. | Stats | | 8. Antony James |  GBR | 53.25s | +1.53s | 07 | 31. | Stats |
| Heat 5 | time | +time | L | Rk. | | | 1. Chad le Clos | RSA | 51.54s | | 07 | 1. | Stats | | 2. Konrad Czerniak | POL | 51.85s | +0.31s | 04 | 4. | Stats | | 3. Steffen Deibler | GER | 51.92s | +0.38s | 06 | 6. | Stats | | 4. Jason Dunford | KEN | 52.23s | +0.69s | 05 | 15. | Stats | | 5. Ivan Lender | SRB | 52.40s | +0.86s | 01 | 18. | Stats | | 6. Takuro Fujii | JPN | 52.49s | +0.95s | 03 | 21. | Stats | | 7. Jayden Hadler | AUS | 52.52s | +0.98s | 02 | 23. | Stats | | 8. Albert Subirats Altes |  VEN | 53.18s | +1.64s | 08 | 29. | Stats |
| Heat 4 | time | +time | L | Rk. | | | 1. Evgeny Korotyshkin | RUS | 51.84s | | 03 | 3. | Stats | | 2. Tyler McGill | USA | 51.95s | +0.11s | 04 | 7. | Stats | | 3. Jiawei Zhou | CHN | 52.06s | +0.22s | 07 | 8. | Stats | | 4. Benjamin Starke | GER | 52.36s | +0.52s | 05 | 16. | Stats | | 5. Lars Frolander |  SWE | 52.47s | +0.63s | 01 | 20. | Stats | | 6. Matteo Rivolta |  ITA | 52.50s | +0.66s | 08 | 22. | Stats | | 7. Michael Rock | GBR | 52.56s | +0.72s | 06 | 24. | Stats | | 8. Kaio Almeida |  BRA | 53.14s | +1.30s | 02 | 28. | Stats |
| Heat 3 | time | +time | L | Rk. | | | 1. Bence Pulai | HUN | 52.19s | | 06 | 12. | Stats | | 2. Dinko Jukic | AUT | 52.22s | +0.03s | 07 | 13. | Stats | | 3. Peter Mankoc |  SLO | 52.44s | +0.25s | 05 | 19. | Stats | | 4. Ryan Pini |  PNG | 52.68s | +0.49s | 02 | 25. | Stats | | 5. Gyucheol Chang |  KOR | 52.69s | +0.50s | 04 | 26. | Stats | | 6. Joe Bartoch |  CAN | 53.09s | +0.90s | 01 | 27. | Stats | | 7. Clement Lefert |  FRA | 53.22s | +1.03s | 03 | 30. | Stats | | 8. Simao Morgado |  POR | 53.26s | +1.07s | 08 | 32. | Stats |
| Heat 2 | time | +time | L | Rk. | | | 1. Dominik Meichtry |  SUI | 53.40s | | 07 | 33. | Stats | | 2. Pavel Sankovich |  BLR | 53.47s | +0.07s | 04 | 34. | Stats | | 3. Joseph Schooling |  SIN | 53.63s | +0.23s | 05 | 35. | Stats | | 4. Benjamin Hockin Brusquetti |  PAR | 53.65s | +0.25s | 03 | 36. | Stats | | 5. Daniel Bell |  NZL | 53.76s | +0.36s | 01 | 37. | Stats | | 6. Yevgeniy Lazuka |  AZE | 53.86s | +0.46s | 08 | 38. | Stats | | 7. Vytautas Janusaitis |  LTU | 54.17s | +0.77s | 06 | 39. | Stats | | 8. Stefanos Dimitriadis |  GRE | 54.20s | +0.80s | 02 | 40. | Stats |
| Heat 1 | time | +time | L | Rk. | | | 1. El-Gadi Sofyan |  LBA | 56.99s | | 04 | 41. | Stats | | 2. Mohanad Ahmed Dheyaa Al-Azzawi |  IRQ | 1:00.71s | +3.72s | 05 | 42. | Stats | | 3. Khalid Alibaba |  BRN | 1:04.05s | +7.06s | 03 | 43. | Stats |
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| Total eligible to participate 44 swimmers. Started 43. |
| Olympic Qualifying Time - 52.36s - 16+7 | | Chris Wright | AUS | 11. | Jayden Hadler | AUS | 23. | | Steffen Deibler | GER | 4. | Benjamin Starke | GER | 14. | | Antony James | GBR | 31. | Michael Rock | GBR | 24. | | Takuro Fujii | JPN | 21. | Takeshi Matsuda | JPN | 17. | | Yevgeny Korotyshkin | RUS | 2. | Nikolay Skvortsov | RUS | 10. | | Milorad Čavić | SRB | 4. | Ivan Lendjer | SRB | 18. | | Michael Phelps | USA | 1. | Tyler McGill | USA | 7. | | Kaio Almeida | BRA | 28. | Glauber Silva | BRA | | | François Heersbrandt | BEL | 16. | | Zhou Jiawei | CHN | 13. | | Jason Dunford | KEN | 12. | | Joeri Verlinden | NED | 6. | | Konrad Czerniak | POL | 8. | | Chad Le Clos | RSA | 2. | | Lars Frölander | SWE | 20. |
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| Olympic Selection Time - 54.19s - 18 | | Albert Subirats | VEN | 52.37s | 29. | | Matteo Rivolta | ITA | 52.40s | 22. | | Chang Gyu-Cheol | KOR | 52.45s | 26. | | Peter Mankoč | SLO | 52.46s | 19. | | Clément Lefert | FRA | 52.48s | 30. | | Bence Pulai | HUN | 52.56s | 14. | | Ryan Pini | PNG | up | 25. | | Dinko Jukic | AUT | qoe | 9. | | Joe Bartoch | CAN | 52.90s | 27. | | Simão Morgado | POR | 52.95s | 32. | | Shaune Fraser | CAY | qoe | | | Pavel Sankovich | BLR | 52.97s | 34. | | Benjamin Hockin | PAR | qoe | 36. | | Joseph Schooling | SIN | qoe | 35. | | Vytautas Janusaitis | LTU | qoe | 39. | | Stefanos Dimitriadis | GRE | qoe | 40. | | Dominik Meichtry | SUI | qoe | 33. | | Daniel Bell | NZL | qoe | 37. |
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| Wildcard Places - 4 | | Yevgeniy Lazuka | AZE | 38. | | Khalid Alibaba | BRN | 43. | | Mohanad Al-Azzawi | IRQ | 42. | | Sofian Elgidi | LBA | 41. |
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| Olympic Qualification time | time | place | date | birth | | 1. Michael Phelps | USA | 50.71s | Shanghai(CHN) | 24.07.2011 | 30/06/1985 | | 2. Konrad Czerniak | POL | 51.15s | Shanghai(CHN) | 24.07.2011 | 11/07/1989 | | 3. Tyler McGill | USA | 51.26s | Shanghai(CHN) | 24.07.2011 | 18/08/1987 | | 4. Milorad Cavic | SRB | 51.45s | Debrecen(HUN) | 21.05.2012 | 31/05/1984 | | 5. Jason Dunford | KEN | 51.59s | Shanghai(CHN) | 24.07.2011 | 28/11/1986 | | 6. Benjamin Starke | GER | 51.65s | Berlin(GER) | 01.06.2011 | 25/11/1986 | | 7. Chris Wright | AUS | 51.67s | Adelaide(AUS) | 15.03.2012 | 07/05/1988 | | 8. Takuro Fujii | JPN | 51.69s | Shanghai(CHN) | 24.07.2011 | 21/04/1985 | | 9. Evgeny Korotyshkin | RUS | 51.77s | Shanghai(CHN) | 24.07.2011 | 30/04/1983 | | 10.Joeri Verlinden | NED | 51.85s | Eindhoven(NED) | 07.04.2011 | 22/01/1988 | | 11.Steffen Deibler | GER | 52.00s | Berlin(GER) | 10.05.2012 | 10/07/1987 | | 12.Michael Rock | GBR | 52.02s | London(GBR) | 03.03.2012 | 13/03/1987 | | 13.Nikolay Skvortsov | RUS | 52.08s | Moscow(RUS) | 17.04.2012 | 28/03/1984 | | 14.Jayden Hadler | AUS | 52.09s | Adelaide(AUS) | 15.03.2012 | 23/09/1993 | | 15.Kaio Almeida | BRA | 52.11s | Rio De Janeiro(BRA) | 03.05.2011 | 19/10/1984 | | 16.Anthony James | GBR | 52.13s | Sheffield(GBR) | 14.06.2011 | 05/11/1989 | | 17.Chad Le Clos | RSA | 52.17s | Maputo(MOZ) | 05.09.2011 | 12/04/1992 | | 18.Jiawei Zhou | CHN | 52.26s | Shaoxing(CHN) | 01.04.2012 | 23/08/1983 | | 19.Francois Heersbrandt | BEL | 52.29s | Antwerpen(BEL) | 20.01.2012 | 12/12/1989 | | 20.Ivan Lendjer | SRB | 52.29s | Istanbul(TUR) | 06.04.2011 | 29/07/1990 | | 21.Lars Froelander | SWE | 52.34s | Shanghai(CHN) | 24.07.2011 | 26/05/1975 | | 22.Takeshi Matsuda | JPN | 52.36s | Tokyo(JPN) | 02.04.2012 | 23/06/1984 | | -. Glauber Silva | BRA | 52.24s | | 12.05.2012 |
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On 27th June 2012 the Brazilian confederation anounced Glauber Silva tested positive for increased levels of testosterone and has been suspended for 2 years.
He tested positive during a competition in May in Rio de Janeiro, which is where he had earned his Olympic spot.
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London 2012