| Swimmer | time | Birth | L | Heat | Fin. |
| 1. Nathan Adrian | USA | 47.52s | 1988 | 4 | Heat 9. | 3. |
| 2. James Magnussen | AUS | 47.53s | 1991 | 4 | Heat 8. | 1. |
| 3. Sebastiaan Verschuren | NED | 47.88s | 1988 | 4 | Heat 7. | 13. |
| 4. Vladimir Morozov | RUS | 47.93s | 1992 | 5 | Heat 9. | 5. |
| 5. Hanser Garcia Hernandez | CUB | 48.04s | 1988 | 5 | Heat 8. | 11. |
| 6. Cameron Mcevoy | AUS | 48.07s | 1994 | 5 | Heat 7. | 4. |
| 7. Marcelo Chierighini | BRA | 48.11s | 1991 | 3 | Heat 9. | 6. |
| 8. Nikita Lobintsev | RUS | 48.17s | 1988 | 3 | Heat 8. | 14. |
| 9. James Feigen | USA | 48.24s | 1989 | 3 | Heat 7. | 3. |
| 10.Luca Dotto | ITA | 48.30s | 1990 | 6 | Heat 9. | 8. |
| 11.Filippo Magnini | ITA | 48.44s | 1982 | 6 | Heat 7. | 16. |
| 11.Konrad Czerniak | POL | 48.44s | 1989 | 6 | Heat 8. | 15. |
| 13.William Meynard | FRA | 48.53s | 1987 | 2 | Heat 9. |
| 14.Adam Brown | GBR | 48.67s | 1989 | 2 | Heat 8. | 9. |
| 15.Fernando Santos | BRA | 48.72s | 1988 | 2 | Heat 7. |
| 16.Fabien Gilot | FRA | 48.74s | 1984 | 7 | Heat 9. | 7. |
| 17.Pieter Timmers | BEL | 48.76s | 1988 | 7 | Heat 8. | 12. |
| 18.Shinri Shioura | JPN | 48.78s | 1991 | 7 | Heat 7. | 10. |
| 19.Zhiwu Lu | CHN | 48.86s | 1989 | 1 | Heat 9. |
| 20.Kenta Ito | JPN | 48.92s | 1990 | 1 | Heat 8. |
| Standard B |
| 21.Norbert Trandafir | ROU | 49.02s | 1988 | 1 | Heat 7. |
| 22.Krisztian Takacs | HUN | 49.35s | 1985 | 8 | Heat 9. |
| 23.Marco Di Carli | GER | 49.37s | 1985 | 8 | Heat 8. |
| 24.Kemal Arda Gurdal | TUR | 49.41s | 1990 | 8 | Heat 7. |
| 25.Leith Shankland | RSA | 49.44s | 1991 | 0 | Heat 8. |
| 26.Martin Verner | CZE | 49.49s | 1980 | 0 | Heat 8. |
| 27.Aitor Martinez Rodriguez | ESP | 49.54s | 1993 | 0 | Heat 6. |
| 28.Benjamin Hockin | PAR | 49.64s | 1986 | 9 | Heat 9. |
| 29.Cristian Quintero | VEN | 49.77s | 1992 | 9 | Heat 7. |
| 29.Sahnoune Oussama | ALG | 49.77s | 1992 | 9 | Heat 8. |
| Outside Best 3 Heats |
| 31.Mindaugas Sadauskas | LTU | 49.78s | 1990 | 4 | Heat 6. |
| 32.Uvis Kalnins | LAT | 49.96s | 1993 | 5 | Heat 6. |
| 33.Federico Grabrich | ARG | 49.97s | 1990 | 3 | Heat 6. |
| 34.Croyeb Gailloty | CAF | 50.00s | 1995 | 6 | Heat 6. |
| 35.Pjotr Degtjarjov | EST | 50.14s | 1993 | 2 | Heat 6. |
| 36.Yauhen Tsurkin | BLR | 50.32s | 1990 | 7 | Heat 6. |
| 37.Joel Greenshields | CAN | 50.36s | 1988 | 1 | Heat 6. |
| 38.Mario Todorovic | CRO | 50.38s | 1988 | 8 | Heat 6. |
| 39.Kai Quan Yeo | SIN | 50.51s | 1990 | 0 | Heat 6. |
| 40.Virdhawal Vikram Khade | IND | 50.53s | 1991 | 9 | Heat 6. |
| 41.Boris Stojanovic | SRB | 50.62s | 1989 | 4 | Heat 5. |
| Outside Qualifiyng Times |
| 42.Geoffrey Robin Cheah | HKG | 50.65s | 1990 | 5 | Heat 5. |
| 43.Triady Fauzi Sidiq | INA | 50.86s | 1991 | 3 | Heat 5. |
| 44.Mohammad Madouh | KUW | 51.12s | | 6 | Heat 5. |
| 45.Joshua Mcleod | TRI | 51.25s | 1990 | 2 | Heat 5. |
| 46.Kevin Avila | GUA | 51.44s | 1992 | 7 | Heat 5. |
| 47.Andrew Chetcuti | MLT | 51.61s | 1992 | 1 | Heat 5. |
| 48.Davit Sikharulidze | GEO | 51.64s | 1984 | 8 | Heat 5. |
| 49.Yu Lian Wang | TPE | 51.71s | 1994 | 0 | Heat 5. |
| 50.Sebastian Jahnsen Madico | PER | 51.88s | 1990 | 9 | Heat 5. |
| 51.El Hazzaz Mehdi | MAR | 51.93s | 1991 | 4 | Heat 4. |
| 52.Enzo Martinez | URU | 51.99s | 1994 | 3 | Heat 4. |
| 52.Jemal Emmanuel Le Grand | ARU | 51.99s | 1994 | 5 | Heat 4. |
| 54.Jessie Khing Lacuna | PHI | 52.13s | 1993 | 6 | Heat 4. |
| 55.Andrew Mark Rutherfurd Aliaga | BOL | 52.42s | 1988 | 2 | Heat 4. |
| 56.Roy-Allan Burch | BER | 52.45s | 1985 | 7 | Heat 4. |
| 57.Abdoul Khadre Mbaye Niane | SEN | 52.57s | 1988 | 1 | Heat 4. |
| 58.Jose Montoya | CRC | 52.83s | 1991 | 8 | Heat 4. |
| 59.Sean Gunn | ZIM | 52.86s | 1993 | 0 | Heat 4. |
| 60.Allan Gabriel Gutierrez Castro | HON | 53.10s | 1993 | 9 | Heat 4. |
| 61.Vahan Mkhitaryan | ARM | 53.11s | 1996 | 4 | Heat 3. |
| 62.Anthony Clark | TAH | 53.16s | 1991 | 5 | Heat 3. |
| 63.Esau Simpson | GRN | 53.26s | 1990 | 3 | Heat 3. |
| 64.Arsham Mirzaei | IRI | 53.50s | 1995 | 6 | Heat 3. |
| 65.Joshua Runako Daniel | LCA | 53.51s | 1995 | 2 | Heat 3. |
| 66.Cheok Fong Lei | MAC | 53.96s | 1990 | 7 | Heat 3. |
| 67.Christopher Duenas | GUM | 54.00s | 1991 | 1 | Heat 3. |
| 68.Ahmed Al-Dulaimi | IRQ | 54.23s | 1992 | 8 | Heat 3. |
| 69.Sergey Krovyakov | TKM | 54.43s | 1991 | 0 | Heat 3. |
| 70.Samson Opuakpo Forcados | NGR | 55.04s | 1986 | 9 | Heat 3. |
| 71.Christian Nazario Nikles | BRU | 55.55s | 1997 | 4 | Heat 2. |
| 72.D. Kalu Achchige | SRI | 55.56s | 1994 | 5 | Heat 2. |
| 73.Farhan Saleh Faraj Sultan | BRN | 55.76s | 1996 | 3 | Heat 2. |
| 74.Stanford Gore Kawale | PNG | 56.17s | 1995 | 6 | Heat 2. |
| 75.Noah Mascoll-Gomes | ANT | 56.99s | 1999 | 2 | Heat 2. |
| 76.Zandanbal Gunsennorov | MGL | 57.12s | 1996 | 7 | Heat 2. |
| 77.Syed Mazhar Hussain Naqvi | PAK | 58.25s | 1993 | 1 | Heat 2. |
| 78.Pathana Inthavong | LAO | 59.42s | 1997 | 8 | Heat 2. |
| 79.Sylla Alassane | CIV | 59.75s | 1995 | 0 | Heat 2. |
| 80.Ammaar Ghadiyali | TAN | 1:00.56 | 1997 | 9 | Heat 2. |
| 81.Giordan Harris | MHL | 1:00.68 | 1993 | 4 | Heat 1. |
| 82.Sirish Gurung | NEP | 1:01.00 | 1998 | 5 | Heat 1. |
| 83.Billy-Scott Irakoze | BDI | 1:02.96 | 1996 | 3 | Heat 1. |
| 84.Kwesi Abbiw Jackson | GHA | 1:03.08 | 1998 | 6 | Heat 1. |
| 85.Robel Kiros Habte | ETH | 1:05.05 | 1992 | 2 | Heat 1. |
| 86.Ibrahima Ela Camara | GUI | 1:06.55 | 1988 | 7 | Heat 1. |
| 87.Shawn Erbelau Dingilius Wallace | PLW | 1:07.62 | 1994 | 1 | Heat 1. |
| 88.Tevoedjre Godonou Wilfrid | BEN | 1:08.00 | 1979 | 8 | Heat 1. |
| 89.Bradley Vincent | MRI | | 1991 | 9 | Heat 1. |
| 90.Abdulrahman Al-Ishaq | QAT | | 1993 | 0 | Heat 1. |