| Swimmer | time | Birth | L | Heat | Fin. |
| 1. Cate Campbell | AUS | 52.83s | 1992 | 4 | Heat 8. | 1. |
| 2. Ranomi Kromowidjojo | NED | 53.00s | 1990 | 4 | Heat 7. | 3. |
| 3. Yi Tang | CHN | 53.28s | 1993 | 4 | Heat 6. | 7. |
| 4. Missy Franklin | USA | 53.43s | 1995 | 5 | Heat 8. | 4. |
| 5. Jeanette Ottesen Gray | DEN | 53.51s | 1987 | 5 | Heat 6. | DNS |
| 6. Camille Muffat | FRA | 53.51s | 1989 | 5 | Heat 7. | DNS |
| 7. Sarah Sjostrom | SWE | 53.66s | 1993 | 3 | Heat 8. | 2. |
| 8. Bronte Campbell | AUS | 53.72s | 1994 | 3 | Heat 7. | 11. |
| 9. Arianna Vanderpool-Wallace | BAH | 53.73s | 1990 | 3 | Heat 6. | 10. |
| 10.Shannon Vreeland | USA | 53.83s | 1991 | 6 | Heat 8. | 8. |
| 11.Femke Heemskerk | NED | 53.88s | 1987 | 6 | Heat 7. | 5. |
| 12.Britta Steffen | GER | 54.05s | 1983 | 6 | Heat 6. | 6. |
| 13.Veronika Popova | RUS | 54.25s | 1991 | 2 | Heat 8. | 9. |
| 14.Michelle Coleman | SWE | 54.26s | 1993 | 2 | Heat 7. | 12. |
| 15.Amy Smith | GBR | 54.27s | 1987 | 2 | Heat 6. |
| 16.Daniela Schreiber | GER | 54.31s | 1989 | 7 | Heat 8. |
| 17.Haruka Ueda | JPN | 54.35s | 1988 | 7 | Heat 7. |
| 18.Pernille Blume | DEN | 54.44s | 1994 | 7 | Heat 6. | 13. |
| 19.Charlotte Bonnet | FRA | 54.45s | 1995 | 1 | Heat 8. |
| 20.Yuhan Qiu | CHN | 54.64s | 1998 | 1 | Heat 7. | 16. |
| 21.Cheuk Yuen Victoria Poon | CAN | 54.67s | 1984 | 1 | Heat 6. |
| Standard B |
| 22.Hanna-Maria Seppala | FIN | 54.93s | 1984 | 8 | Heat 8. |
| 23.Evelin Verraszto | HUN | 54.93s | 1989 | 8 | Heat 7. | 15. |
| 24.Melanie Costa Schmid | ESP | 55.15s | 1989 | 8 | Heat 6. |
| 25.Alessandra Marchioro | BRA | 55.15s | 1993 | 0 | Heat 8. |
| 26.Ruta Meilutyte | LTU | 55.28s | 1997 | 0 | Heat 6. |
| 27.Erika Ferraioli | ITA | 55.32s | 1986 | 0 | Heat 6. |
| 28.Nina Rangelova | BUL | 55.52s | 1990 | 9 | Heat 8. |
| 29.Liliana Ibanez Lopez | MEX | 55.71s | 1991 | 9 | Heat 7. |
| 30.Aksana Dziamidava | BLR | 55.86s | 1993 | 9 | Heat 6. |
| Outside Best 3 Heats |
| 31.Kimberly Buys | BEL | 55.89s | 1989 | 4 | Heat 5. |
| 32.Elmira Aigaliyeva | KAZ | 55.97s | 1992 | 5 | Heat 5. |
| 33.Julie Meynen | LUX | 55.98s | 1997 | 3 | Heat 5. |
| 34.Samantha Lucie-Smith | NZL | 55.99s | 1992 | 6 | Heat 5. |
| 35.Karin Prinsloo | RSA | 56.14s | 1989 | 2 | Heat 5. | 14. |
| 36.Ting Wen Quah | SIN | 56.14s | 1992 | 7 | Heat 5. |
| 37.Miroslava Najdanovski | SRB | 56.22s | 1988 | 1 | Heat 5. |
| 38.Isabella Arcila Hurtado | COL | 56.53s | 1994 | 8 | Heat 5. |
| 39.Susann Bjornsen | NOR | 56.63s | 1993 | 0 | Heat 5. |
| 40.Farida Osman | EGY | 56.72s | 1995 | 9 | Heat 5. |
| 41.Sycerika Mcmahon | IRL | 56.74s | 1995 | 4 | Heat 4. |
| 42.Sabina Dostalova | CZE | 56.77s | 1990 | 5 | Heat 4. |
| Outside Qualifiyng Times |
| 43.Jasmine Alkhaldi | PHI | 56.92s | 1993 | 3 | Heat 4. |
| 44.Anastasia Katerina Bogdanovski | MKD | 57.22s | 1993 | 6 | Heat 4. |
| 45.Benjaporn Sriphanomthorn | THA | 57.25s | 1995 | 2 | Heat 4. |
| 46.Alexia Pamela Benitez Quijada | ESA | 57.65s | 1991 | 7 | Heat 4. |
| 47.Karen Riveros Schulz | PAR | 57.71s | 1994 | 1 | Heat 4. |
| 48.Karen Milenka Torrez Guzman | BOL | 57.82s | 1992 | 8 | Heat 4. |
| 49.Elisbet Gamez Matos | CUB | 58.00s | 1997 | 0 | Heat 4. |
| 50.Loren Yamile Bahamonde Cabello | ECU | 58.01s | 1987 | 9 | Heat 4. |
| 51.Bayan Jumah | SYR | 58.14s | 1994 | 4 | Heat 3. |
| 52.Allyson Roxanne Ponson | ARU | 58.28s | 1995 | 5 | Heat 3. |
| 53.Monika Vasilyan | ARM | 58.50s | 1995 | 3 | Heat 3. |
| 54.Sylvia Brunlehner | KEN | 58.77s | 1994 | 6 | Heat 3. |
| 55.Chinyere Pigot | SUR | 58.97s | 1993 | 2 | Heat 3. |
| 56.Julimar Cecilia Avila Mancia | HON | 58.98s | 1997 | 7 | Heat 3. |
| 57.Monica Ramirez Abella | AND | 1:00.04 | 1993 | 1 | Heat 3. |
| 58.Ana Nobrega | ANG | 1:00.12 | 1990 | 8 | Heat 3. |
| 59.Jamila Nsibambi Lunkuse | UGA | 1:00.48 | 1997 | 0 | Heat 3. |
| 60.Rebecca Heyliger | BER | 1:01.39 | 1992 | 9 | Heat 3. |
| 61.Britany Candacia Van Lange | GUY | 1:01.62 | 1996 | 4 | Heat 2. |
| 62.Sameera Albitar | BRN | 1:04.32 | 1990 | 5 | Heat 2. |
| 63.Tiara Eleina Shahril Anwar | BRU | 1:04.85 | 1997 | 3 | Heat 2. |
| 64.Merjen Saryyeva | TKM | 1:06.38 | 1997 | 6 | Heat 2. |
| 65.Rachel Wall | ANT | 1:06.56 | 1998 | 2 | Heat 2. |
| 66.Charissa Sofia Panuve | TGA | 1:08.31 | 1994 | 7 | Heat 2. |
| 67.Dirngulbai Moreyna Misech | PLW | 1:09.02 | 1997 | 1 | Heat 2. |
| 68.Shreya Dhital | NEP | 1:09.82 | 1995 | 8 | Heat 2. |
| 69.Vivie Geneva Chamberlain | LAO | 1:16.06 | 2001 | 0 | Heat 2. |
| 70.Elsie Uwamahoro | BDI | 1:16.10 | 1988 | 9 | Heat 2. |
| 71.Karina Klimyk | TJK | 1:19.27 | 1999 | 4 | Heat 1. |
| 72.Nazlati Mohamed | COM | 1:21.54 | 1997 | 5 | Heat 1. |
| 73.Heather Ann Arseth | MRI | | 1993 | 6 | Heat 1. |
| 74.Sabine Hazboun | PLE | | 1994 | 3 | Heat 1. |